{"text_format":"3","workbook":"xlsx","n_sheets":7,"sheets":["NGS","Ne pas supprimer","Réservé à PlaGe","Lane","Ressource","Version","Pool Robot"],"not_rendered":{"images":17,"defined_names":7,"comments":2},"read":"whole","date_system":"1900"}

{"sheet":"NGS","index":0,"n_rows":416,"n_cols":18,"rows_kept":416,"merged":[[0,0,0,1],[10,3,10,5],[10,6,10,8],[10,9,10,11],[10,12,10,14],[10,15,10,17],[11,3,11,5],[11,6,11,8],[11,9,11,11],[11,12,11,14],[11,15,11,17],[12,3,12,5],[12,6,12,8],[12,9,12,11],[12,12,12,14],[12,15,12,17],[13,3,13,5],[13,6,13,8],[13,9,13,11],[13,12,13,14],[13,15,13,17],[14,3,14,5],[14,6,14,8],[14,9,14,11],[14,12,14,14],[14,15,14,17],[15,3,15,5],[15,6,15,8],[15,9,15,11],[15,12,15,14],[15,15,15,17],[16,3,16,5],[16,6,16,8],[16,9,16,11],[16,12,16,14],[16,15,16,17],[17,3,17,5],[17,6,17,8],[17,9,17,11],[17,12,17,14],[17,15,17,17],[18,3,18,5],[18,6,18,8],[18,9,18,11],[18,12,18,14],[18,15,18,17],[19,3,19,5],[19,6,19,8],[19,9,19,11],[19,12,19,14],[19,15,19,17],[20,3,20,5],[20,6,20,8],[20,9,20,11],[20,12,20,14],[20,15,20,17],[21,3,21,5],[21,6,21,8],[21,9,21,11],[21,12,21,14],[21,15,21,17],[22,3,22,5],[22,6,22,8],[22,9,22,11],[22,12,22,14],[22,15,22,17],[23,3,23,5],[23,6,23,8],[23,9,23,11],[23,12,23,14],[23,15,23,17],[24,3,24,5],[24,6,24,8],[24,9,24,11],[24,12,24,14],[24,15,24,17],[25,3,25,5],[25,6,25,8],[25,9,25,11],[25,12,25,14],[25,15,25,17],[26,3,26,5],[26,6,26,8],[26,9,26,11],[26,12,26,14],[26,15,26,17],[27,3,27,5],[27,6,27,8],[27,9,27,11],[27,12,27,14],[27,15,27,17],[28,3,28,5],[28,6,28,8],[28,9,28,11],[28,12,28,14],[28,15,28,17]]}
["Technique"]
["Machine"]
["Read type"]
["read 1 Length",null,"Number between 0 and 300"]
["read 2 Length",null,"Number between  0 and 300"]
["Index 1 length",null,"Number between 1 and 18"]
["Index 2 length",null,"Number between 0 and 18"]
["Lane quantity"]
[]
[]
[null,null,"Lane",1,null,null,2,null,3,3,null,5,4,null,7,5]
[null,null,"Laboratory"]
[null,null,"Team"]
[null,null,"Project"]
[null,null,"Lane name"]
[null,null,"Starting material"]
[null,null,"Starting material description",{"$f":"IFERROR(INDEX(Ressource!$A$3:$B$11,MATCH(D16,Ressource!$A$3:$A$11,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$A$3:$B$11,MATCH(G16,Ressource!$A$3:$A$11,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$A$3:$B$11,MATCH(J16,Ressource!$A$3:$A$11,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$A$3:$B$11,MATCH(M16,Ressource!$A$3:$A$11,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$A$3:$B$11,MATCH(P16,Ressource!$A$3:$A$11,0),2),\"\")","v":""}]
[null,null,"Library type"]
[null,null,"Library type description",{"$f":"IFERROR(INDEX(Ressource!$E$3:$F$38,MATCH(D18,Ressource!$E$3:$E$38,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$E$3:$F$38,MATCH(G18,Ressource!$E$3:$E$38,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$E$3:$F$38,MATCH(J18,Ressource!$E$3:$E$38,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$E$3:$F$38,MATCH(M18,Ressource!$E$3:$E$38,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$E$3:$F$38,MATCH(P18,Ressource!$E$3:$E$38,0),2),\"\")","v":""}]
[null,null,"Library selection"]
[null,null,"Library selection description",{"$f":"IFERROR(INDEX(Ressource!$C$3:$D$33,MATCH(D20,Ressource!$C$3:$C$33,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$C$3:$D$33,MATCH(G20,Ressource!$C$3:$C$33,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$C$3:$D$33,MATCH(J20,Ressource!$C$3:$C$33,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$C$3:$D$33,MATCH(M20,Ressource!$C$3:$C$33,0),2),\"\")","v":""},null,null,{"$f":"IFERROR(INDEX(Ressource!$C$3:$D$33,MATCH(P20,Ressource!$C$3:$C$33,0),2),\"\")","v":""}]
[null,null,"Pool concentration (nM)"]
[null,null,"Library average size (pb)"]
[null,null,"Library diversity"]
[null,null,"Specie (Scientific name)"]
[null,null,"Taxonomy Id",{"$f":"getTaxonomicId(D25)","v":"Species not specified"},null,null,{"$f":"getTaxonomicId(G25)","v":"Species not specified"},null,null,{"$f":"getTaxonomicId(J25)","v":"Species not specified"},null,null,{"$f":"getTaxonomicId(M25)","v":"Species not specified"},null,null,{"$f":"getTaxonomicId(P25)","v":"Species not specified"}]
[null,null,"Indexing type"]
[null,null,"Index kit name"]
[null,null,"If other"]
[]
["Index name"," i7-i5 sequences","Sequence OK ?","Library name","C° (nM)","Library size (pb)","Library name","C° (nM)","Library size (pb)","Library name","C° (nM)","Library size (pb)","Library name","C° (nM)","Library size (pb)","Library name","C° (nM)","Library size (pb)"]
["No Index"]
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["Type of DNA or RNA used in the experiment",null,"Whether the source material was selected for certain properties (for\nexample methyl filtrated)",null,"The sequencing technique intended for the library."]
["library source","library source explanation","library selection","library selection explanation","Library strategy","Library strategy explanation"]
["GENOMIC"," Genomic DNA (includes PCR products from genomic DNA).","5-methylcytidine antibody"," Selection of methylated DNA fragments using an antibody raised against 5-methylcytosine or 5-methylcytidine (m5C).","AMPLICON"," Sequencing of overlapping or distinct PCR or RT-PCR products. For example, metagenomic community profiling using SSU rRNA."]
["GENOMIC SINGLE CELL","No description","CAGE"," Cap-analysis gene expression.","ATAC-seq"," Assay for Transposase-Accessible Chromatin (ATAC) strategy is used to study genome-wide chromatin accessibility. alternative method to DNase-seq that uses an engineered Tn5 transposase to cleave DNA and to integrate primer DNA sequences into the cleaved genomic DNA."]
["METAGENOMIC"," Mixed material from metagenome.","cDNA"," PolyA selection or enrichment for messenger RNA (mRNA); synonymize with PolyA","Bisulfite-Seq"," MethylC-seq. Sequencing following treatment of DNA with bisulfite to convert cytosine residues to uracil depending on methylation status."]
["METATRANSCRIPTOMIC"," Transcription products from community targets","cDNA_oligo_dT","No description","ChIA-PET"," Direct sequencing of proximity-ligated chromatin immunoprecipitates."]
["SYNTHETIC"," Synthetic DNA.","cDNA_randomPriming","No description","ChIP-Seq"," ChIP-seq, Chromatin ImmunoPrecipitation, reveals binding sites of specific proteins, typically transcription factors (TFs) using antibodies to extract DNA fragments bound to the target protein."]
["TRANSCRIPTOMIC"," Transcription products or non genomic DNA (EST, cDNA, RT-PCR, screened libraries).","ChIP"," Chromatin immunoprecipitation","CLONE"," Genomic clone based (hierarchical) sequencing."]
["TRANSCRIPTOMIC SINGLE CELL","No description","ChIP-Seq"," Chromatin immunoPrecipitation, reveals binding sites of specific proteins, typically transcription factors (TFs) using antibodies to extract DNA fragments bound to the target protein.","CLONEEND"," Clone end (5’, 3’, or both) sequencing."]
["VIRAL RNA"," Viral RNA.","DNase"," DNase I endonuclease digestion and size selection reveals regions of chromatin where the DNA is highly sensitive to DNase I.","CTS"," Concatenated Tag Sequencing"]
["OTHER"," Other, unspecified, or unknown library source material.","HMPR"," Hypo-methylated partial restriction digest","DNase-Hypersensitivity"," Sequencing of hypersensitive sites, or segments of open chromatin that are more readily cleaved by DNaseI."]
[null,null,"Hybrid Selection"," Selection by hybridization in array or solution.","EST"," Single pass sequencing of cDNA templates"]
[null,null,"Inverse rRNA"," depletion of ribosomal RNA by oligo hybridization.","FAIRE-seq"," Formaldehyde Assisted Isolation of Regulatory Elements. Reveals regions of open chromatin."]
[null,null,"Inverse rRNA selection"," depletion of ribosomal RNA by inverse oligo hybridization.","FINISHING"," Sequencing intended to finish (close) gaps in existing coverage."]
[null,null,"MBD2 protein methyl-CpG binding domain"," Enrichment by methyl-CpG binding domain.","FL-cDNA"," Full-length sequencing of cDNA templates"]
[null,null,"MDA"," Multiple Displacement Amplification, a non-PCR based DNA amplification technique that amplifies a minute quantifies of DNA to levels suitable for genomic analysis.","Hi-C"," Chromosome Conformation Capture technique where a biotin-labeled nucleotide is incorporated at the ligation junction, enabling selective purification of chimeric DNA ligation junctions followed by deep sequencing."]
[null,null,"MF"," Methyl Filtrated","MBD-Seq"," Methyl CpG Binding Domain Sequencing."]
[null,null,"MNase"," Identifies well-positioned nucleosomes. uses Micrococcal Nuclease (MNase) is an endo-exonuclease that processively digests DNA until an obstruction, such as a nucleosome, is reached.","MeDIP-Seq"," Methylated DNA Immunoprecipitation Sequencing."]
[null,null,"MSLL"," Methylation Spanning Linking Library","miRNA-Seq"," Micro RNA sequencing strategy designed to capture post-transcriptional RNA elements and include non-coding functional elements. (see pubmed 21787409 for details)"]
[null,null,"Oligo-dT"," enrichment of messenger RNA (mRNA) by hybridization to Oligo-dT.","MNase-Seq"," Identifies well-positioned nucleosomes. uses Micrococcal Nuclease (MNase) is an endo-exonuclease that processively digests DNA until an obstruction, such as a nucleosome, is reached."]
[null,null,"padlock probes capture method"," Targeted sequence capture protocol covering an arbitrary set of nonrepetitive genomics targets. An example is capture bisulfite sequencing using padlock probes (BSPP).","MRE-Seq"," Methylation-Sensitive Restriction Enzyme Sequencing."]
[null,null,"PCR"," target enrichment via PCR","ncRNA-Seq"," Capture of other non-coding RNA types, including post-translation modification types such as snRNA (small nuclear RNA) or snoRNA (small nucleolar RNA), or expression regulation types such as siRNA (small interfering RNA) or piRNA/piwi/RNA (piwi-interacting RNA)."]
[null,null,"PolyA"," PolyA selection or enrichment for messenger RNA (mRNA); should replace cDNA enumeration.","POOLCLONE"," Shotgun of pooled clones (usually BACs and Fosmids)."]
[null,null,"RACE"," Rapid Amplification of cDNA Ends.","RAD-Seq","No description"]
[null,null,"RANDOM"," No Selection or Random selection","RIP-Seq"," Direct sequencing of RNA immunoprecipitates (includes CLIP-Seq, HITS-CLIP and PAR-CLIP)."]
[null,null,"RANDOM PCR"," Source material was selected by randomly generated primers.","RNA-Seq"," Random sequencing of whole transcriptome, also known as Whole Transcriptome Shotgun Sequencing, or WTSS). (see pubmed 18611170 for details)"]
[null,null,"Reduced Representation"," Reproducible genomic subsets, often generated by restriction fragment size selection, containing a manageable number of loci to facilitate re-sampling.","SELEX"," Systematic Evolution of Ligands by Exponential enrichment"]
[null,null,"repeat fractionation"," Selection for less repetitive (and more gene rich) sequence through Cot filtration (CF) or other fractionation techniques based on DNA kinetics.","ssRNA-seq"," Strand-specific RNA sequencing."]
[null,null,"Restriction Digest"," DNA fractionation using restriction enzymes.","Synthetic-Long-Read"," binning and barcoding of large DNA fragments to facilitate assembly of the fragment"]
[null,null,"RT-PCR"," target enrichment via","Targeted-Capture"," Enrichment of a targeted subset of loci."]
[null,null,"size fractionation"," Physical selection of size appropriate targets.","Tethered Chromatin Conformation Capture"]
[null,null,"other"," Other library enrichment, screening, or selection process.","Tn-Seq"," Quantitatively determine fitness of bacterial genes based on how many times a purposely seeded transposon gets inserted into each gene of a colony after some time."]
[null,null,"unspecified"," Library enrichment, screening, or selection is not specified.","VALIDATION","  CGHub special request:  Independent experiment to re-evaluate putative variants."]
[{"$nulls":4},"WCS"," Random sequencing of a whole chromosome or other replicon isolated from a genome."]
[{"$nulls":4},"WGA"," Whole Genome Amplification followed by random sequencing. (see pubmed 1631067,8962113 for details)"]
[{"$nulls":4},"WGS"," Whole Genome Sequencing - random sequencing of the whole genome (see pubmed 10731132 for details)"]
[{"$nulls":4},"WXS"," Random sequencing of exonic regions selected from the genome. (see pubmed 20111037 for details)"]
[{"$nulls":4},"OTHER"," Library strategy not listed."]

{"sheet":"Version","index":5,"n_rows":4,"n_cols":3,"rows_kept":4}
["date","quoi","qui"]
["2023-07-27T09:03:00","ajout infos pour metadata ENA (lib, espèce, taxo)","Gérald"]
["2023-08-29T16:34:00","ajout possibilité de créer des worklists\najout liens vers ENA\ncalcul automatique de la taille moyenne d'insert du pool","Gérald"]
["2023-09-18T09:09:00","translate to english\nupdate macro to manage english version","Rachel/Eden\nGérald"]

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["SourceLabwareLabel","Source Position","Volumes","Destination Labware Label","Destination Position"]